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http://digitalrepository.fccollege.edu.pk/handle/123456789/1222
Title: | Argemone mexicana extract alleviates gastrointestinal disorders by stimulating muscarinic receptors and blocking voltage-gated L-type calcium channels |
Authors: | Iqbal, Rabia Hamid, Irfan Janbaz, Khalid Hussain Akhtar, Muhammad Furqan Saleem, Ammara Sharif, Ali Peerzada, Sohaib Akhtar, Bushra Sohail, Kashif Ali, Sajid |
Keywords: | Emesis Argemone mexicana; Muscarinic agonist; Calcium channel blocker ; Constipation; |
Issue Date: | Apr-2021 |
Publisher: | www.apjtb.org |
Citation: | TY - JOUR AU - Iqbal, Rabia AU - Hamid, Irfan AU - Janbaz, Khalid AU - Akhtar, Muhammad Furqan AU - Saleem, Ammara AU - Sharif, Ali AU - Peerzada, Sohaib AU - Akhtar, Bushra AU - Sohail, Kashif AU - PY - 2021/04/06 SP - 214 EP - 221 T1 - Argemone mexicana extract alleviates gastrointestinal disorders by stimulating muscarinic receptors and blocking voltage-gated L-type calcium channels VL - 11 DO - 10.4103/2221-1691.311769 JO - Asian Pacific Journal of Tropical Biomedicine ER - |
Series/Report no.: | Asian Pacific Journal of Tropical Biomedicine 2021; 11(5): 214-221; |
Abstract: | Objective: To investigate the pharmacological potential of Argemone mexicana in treating constipation and emesis by using in vitro and in vivo models. Methods: The spasmogenic and spasmolytic effects were evaluated on isolated rabbit jejunum fragments loaded in a tissue organ bath. The response was recorded with an isotonic transducer attached with Power Lab Data Acquisition System. The laxative and antiemetic activities were assessed in BALB-c mice and poultry chicks challenged with carbamylcholine and copper sulphate stimulated emesis, respectively. Results: The total phenolic and total flavonoids contents of the extract were (267.75 ± 5.77) mg GAE/g and (73.86 ± 6.01) mg QE/g, respectively. Argemone mexicana extract exerted spasmogenic effect on isolated rabbit jejunum segments with an EC50 value of 0.016 mg/ mL, which was blocked by atropine (0.3 µM). Argemone mexicana extract exerted spasmolytic effect in atropine treated jejunum fragments with an EC50 value of 2.185 mg/mL. Furthermore, Argemone mexicana extract relaxed potassium (80 mM)-induced contractions (EC50: 9.07 mg/mL), similar to a standard drug verapamil. The calcium channel blocker activity was confirmed by a rightward shift of concentration-response curve of calcium in the presence of Argemone mexicana extract (1-5 mg/mL) and verapamil (0.1-1 µM). In addition, the extract increased the distance travelled by a charcoal in the gastrointestinal tract and exhibited antiemetic effect on copper sulphate induced emesis in chicks. Conclusions: Argemone mexicana shows cholinergic agonist and calcium channel blocker activities, as well as antiemetic effect. It may be used as a potential agent for treating gastrointestinal disorders. |
Description: | https://www.researchgate.net/publication/350655403_Argemone_mexicana_extract_alleviates_gastrointestinal_disorders_by_stimulating_muscarinic_receptors_and_blocking_voltage-gated_L-type_calcium_channels |
URI: | http://localhost:8080/xmlui/handle/123456789/1222 |
Appears in Collections: | Pharmacy Department |
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File | Description | Size | Format | |
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Argemone mexicana extract alleviates gastrointestinal disorders by stimulating muscarinic.pdf | 1.47 MB | Adobe PDF | View/Open |
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