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http://digitalrepository.fccollege.edu.pk/handle/123456789/1235
Title: | Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes |
Authors: | Ambreen, Ghazala Duse, Lili Tariq, Imran Ali, Uzma Ali, Sajid Pinnapireddy, Shashank R. Bette, Michael Bakowsky, Udo Mandic, Robert |
Keywords: | papilloma virus : head and neck squamous cell carcinoma (HNSCC); head and neck cancer; cervical cancer ; photodynamic therapy; curcumin liposomes; |
Issue Date: | 5-Nov-2020 |
Publisher: | MDPI |
Citation: | Ambreen G, Duse L, Tariq I, Ali U, Ali S, Pinnapireddy SR, Bette M, Bakowsky U, Mandic R. Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes. Cancers. 2020; 12(11):3278. https://doi.org/10.3390/cancers12113278 |
Series/Report no.: | Cancers 12, no. 11: 3278.; |
Abstract: | Photodynamic therapy (PDT) is a minimally invasive therapeutic approach used in the treatment of various medical conditions and cancerous diseases, involving light, a photosensitizing substance, and oxygen. Curcumin, a naturally occurring compound, carries antitumor activities and potentially could be exploited as a photosensitizer in PDT. Only little is known about liposomal-encapsulated curcumin that could help in increasing the efficacy, stability, and bioavailability of this compound. This study investigates the in vitro effects of curcumin-loaded liposomes in combination with PDT. Three papilloma virus-associated cell lines were treated with curcumin-loaded liposomes corresponding to a curcumin concentration of 0–100 µmol/L for 4 h followed by illumination at 457 nm (blue) for 45, 136, and 227 s at a fluence of 220.2 W/m2 (100 mA) corresponding to 1, 3 and 5 J·cm−2 . After 24 h, the biological outcome of the treatment was assessed with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), SYTO9/PI (propidium iodide), Annexin V-FITC (fluorescein isothiocyanate)/PI, clonogenic survival, and scratch (wound closure) assays. Photoactivation of curcumin-loaded liposomes led to a significant reduction in colony formation and migratory abilities, as well as to an increase in tumor cell death. The results point to Cancers 2020, 12, 3278; doi:10.3390/cancers12113278 www.mdpi.com/journal/cancersCancers 2020, 12, 3278 2 of 16 the combination of curcumin-loaded liposomes with PDT as a potentially useful tool for the treatment of papillomavirus-associated malignancies. |
Description: | https://www.mdpi.com/2072-6694/12/11/3278 |
URI: | http://localhost:8080/xmlui/handle/123456789/1235 |
Appears in Collections: | Pharmacy Department |
Files in This Item:
File | Description | Size | Format | |
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Sensitivity of Papilloma Virus-Associated Cell Lines.pdf | 5.05 MB | Adobe PDF | View/Open |
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