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Title: | Selective anti-ErbB3 aptamer modified sorafenib microparticles: In vitro and in vivo toxicity assessment |
Authors: | Ali, Muhammad Yasir Sohail, Muhammad Farhan Amin, Muhammad Umair Tariq, Imran Ali, Sajid Pinnapireddy, Shashank Reddy Ali, Asad Schäfer, Jens Bakowsky, Udo |
Keywords: | Cancer Polymer PLGA CLSM SEM |
Issue Date: | Dec-2019 |
Publisher: | ELSEVIER |
Citation: | Muhammad Yasir Ali, Imran Tariq, Muhammad Farhan Sohail, Muhammad Umair Amin, Sajid Ali, Shashank Reddy Pinnapireddy, Asad Ali, Jens Schäfer, Udo Bakowsky, Selective anti-ErbB3 aptamer modified sorafenib microparticles: In vitro and in vivo toxicity assessment, European Journal of Pharmaceutics and Biopharmaceutics, Volume 145, 2019, Pages 42-53, ISSN 0939-6411, https://doi.org/10.1016/j.ejpb.2019.10.003. |
Series/Report no.: | European Journal of Pharmaceutics and Biopharmaceutics, Volume 145, 2019, Pages 42-53,; |
Abstract: | The delivery of aptamer modified therapeutic moieties to specific tissue sites has become one of the major therapeutic choices to reduce the toxicity of inhibitory drugs. Bearing this in mind, the current study was de signed using sorafenib (SFB) encapsulated microparticles (MP) prepared with biodegradable poly (D, L-lactic-co glycolic acid) (PLGA) copolymer. The surfaces of these microparticles were modified with RNA aptamer having a binding affinity towards ErbB3 receptors. SFB-loaded MP (MPS) were prepared by o/w solvent evaporation method and the surface was coupled with the amino group of aptamer by EDC/NHS chemistry. Physiochemical investigations were done by dynamic light scattering, scanning electron microscopy and FTIR. In vitro apoptosis assay, cell viability assay and metastatic progression showed a significant decrease (p < 0.001) in vitro cell viability for MPS and MPS-Apt as compared to MP. The synergistic combination of SFB and aptamer also de creased the metastatic progression of cells for an extended period. Microparticles were also evaluated for in vivo toxicity in female BALB/c mice. It was evident that the presence of aptamer decreased the generalized toxicity of MPS-Apt, as measured by mean body weight loss and blood profiles, keeping all the blood formed elements level within acceptable limits. The histopathological investigations showed some necrotic and pyknotic bodies. In a similar fashion, liver function test and renal function tests showed pronounced effects of formulations on vital organs. |
Description: | https://www.sciencedirect.com/science/article/abs/pii/S0939641119309828 |
URI: | http://localhost:8080/xmlui/handle/123456789/1236 |
Appears in Collections: | Pharmacy Department |
Files in This Item:
File | Description | Size | Format | |
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Selective anti-ErbB3 aptamer modified sorafenib microparticles; In vitro and.pdf | 4.47 MB | Adobe PDF | View/Open |
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