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DC Field | Value | Language |
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dc.contributor.author | Tariq, Imran | - |
dc.contributor.author | Pinnapireddy, , Shashank Reddy | - |
dc.contributor.author | Duse, Lili | - |
dc.contributor.author | Ali, Muhammad Yasir | - |
dc.contributor.author | Ali, Sajid | - |
dc.contributor.author | Amin, Muhammad Umair | - |
dc.contributor.author | Goergen, Nathalie | - |
dc.contributor.author | Jedelská, Jarmila | - |
dc.contributor.author | Schäfer, Jens | - |
dc.contributor.author | Bakowsky, Udo | - |
dc.date.accessioned | 2021-04-20T08:58:17Z | - |
dc.date.available | 2021-04-20T08:58:17Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | Imran Tariq, Shashank Reddy Pinnapireddy, Lili Duse, Muhammad Yasir Ali, Sajid Ali, Muhammad Umair Amin, Nathalie Goergen, Jarmila Jedelská, Jens Schäfer, Udo Bakowsky, Lipodendriplexes: A promising nanocarrier for enhanced gene delivery with minimal cytotoxicity, European Journal of Pharmaceutics and Biopharmaceutics, Volume 135, 2019, Pages 72-82, ISSN 0939-6411, https://doi.org/10.1016/j.ejpb.2018.12.013. | en_US |
dc.identifier.other | https://doi.org/10.1016/j.ejpb.2018.12.013 | - |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/1239 | - |
dc.description | https://www.sciencedirect.com/science/article/abs/pii/S093964111831124X | en_US |
dc.description.abstract | Non-viral vectors are a safe, efficient and non-toxic alternative to viral vectors for gene therapy against many diseases ranging from genetic disorders to cancers. Polyamidoamine (PAMAM), a positively charged dendrimer has a tendency to complex with nucleic acids (to form dendriplexes) like plasmid DNA (pDNA) and small in terfering RNA (siRNA) and can shield them from enzymatic degradation, thereby facilitating endocytosis and endosomal release. In this study, we developed an advanced variant of the dendriplexes by encapsulating them within liposomes to enhance their gene delivery efficiency. This liposome encapsulated dendriplex system can further reduce unwanted cytotoxicity and enhance cellular uptake of nucleic acids. A broad range of lipid combinations were used to optimize the lipodendriplexes in terms of their physicochemical characteristics in cluding size, shape and zeta potential. The optimized lipodendriplexes were tested for pDNA transfection, in vitro cell viability, cellular uptake, siRNA mediated knockdown, hemocompatibility, metastatic progression and in ovo in chorioallantoic membrane model (CAM). The optimized system has shown significant improvement in pDNA transfection (p < 0.01) with higher GFP expression and gene silencing and has shown improved cell viability (p < 0.05) compared to the parent dendriplex system. The hemocompatibility and CAM analysis, revealed an efficient yet biocompatible gene delivery system in the form of lipodendriplexes. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartofseries | European Journal of Pharmaceutics and Biopharmaceutics Volume 135, February 2019, Pages 72-82; | - |
dc.subject | Biocompa | en_US |
dc.subject | Liposomes | en_US |
dc.subject | PAMAM | en_US |
dc.subject | Dendriplexes | en_US |
dc.subject | Transfection | en_US |
dc.subject | Gene therapy | en_US |
dc.title | Lipodendriplexes: A promising nanocarrier for enhanced gene delivery with minimal cytotoxicity | en_US |
dc.type | Article | en_US |
Appears in Collections: | Pharmacy Department |
Files in This Item:
File | Description | Size | Format | |
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Lipodendriplexes; A promising nanocarrier for enhanced gene delivery with minimal cytotoxicity.pdf | 10.44 MB | Adobe PDF | View/Open |
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