Assessment of heavy metals and associated oxidative stress in occupationally exposed workers from Bannu and Karak Districts in Pakistan

Abstract

Heavy metals (HMs) are extensively found in occupationally exposed miners and indus trial workers, which may cause serious health-related problems to the large workforce. In order to evaluate the impact of these toxic pollutants, we have inves tigated the efect of cadmium (Cd), chromium (Cr), copper (Cu), and lead (Pb) concentration on exposed workers of mining, and woolen textile mill and com pared the fndings with unexposed individuals. From each category like exposed workers (mining, and woolen mill textile site) and unexposed individuals, 50 blood samples were taken. The occurrence of HMs in a sample was investigated through atomic absorp tion spectrometry while the oxidative stress marker malondialdehyde (MDA) and antioxidant enzyme statuses such as superoxide dismutase (SOD) and catalase (CAT) were analyzed in exposed and control samples. The results showed signifcant (p<0.05) variation in Cd, Cr, Cu, and Pb levels in exposed and control samples. The concentration of Cd in the blood of WMWs, KMWs, and control group was 5.75, 3.89, and 0.42 μg/dL, respectively. On the other hand, the concentration of Pb in the blood of WMWs, MWs, and control was 32.34, 24.39, and 0.39 µg/dL while the concentrations of Cr and Cu in the blood of WMWs, MWs, and control group were 11.61 and 104.14 μg/dL, 4.21 and 113.21 μg/dL, 0.32 and 65.53 μg/dL, respectively. An increase in MDA was recorded in the exposed workers’ group as compared to control subjects, whereas SOD and CAT activities decreased. Meanwhile, MDA was signifcantly and positively (p<0.01) correlated with HMs, while neg ative signifcant correlations were found among HMs with SOD and CAT