Comparative water-use efficiency of Sporobolus arabicus and Leptochloa fusca and its relation with carbon-isotope discrimination under semi-arid conditions

Abstract

Water-use efficiency (WUE) of Leptochloa fusca (L.) Kunth (Kallar grass) and Sporobolus arabicus Boiss. was determined under different soil moisture regimes. Plants grown in lysimeters were subjected to three soil moisture regimes, viz. well-watered (100%), medium-watered (75%), and low-watered (50%) of total available water (TAW). The soil moisture was restored on alternate days by adding the required volume of water on the basis of neutron moisture meter readings taken from neutron access tubes installed in each lysimeter. The grasses were harvested after suitable intervals (∼4 months) to obtain maximum biomass. Leaf samples collected at each harvest were analyzed for carbon-isotope discrimination (δ13C) with an isotope ratio (13C/12C) mass spectrometer. Results indicated significant differences in WUE of both grasses subjected to different water regimes. Sporobolus arabicus showed higher WUE than Kallar grass. However, Kallar grass showed better value of yield response factor (k y = 0.649) compared with Sporobolus (k y = 1.06) over the entire season. The data confirm that these grasses can be grown successfully in water-limited environments by selecting an optimum soil moisture level for maximum biomass production. The mean carbon-isotope discrimination (δ13C) of Kallar grass (−14.4‰) and Sporobolus (−12.8‰) confirm that both are C4 plants. The carbon-isotope discrimination (Δ) was significantly and negatively correlated with WUE of the two species studied. The results of the present study confirm that δ13C or Δ of leaves can be used as good predictor of WUE in some C4 plants.