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Title: | Saccharification of Leptochloa fusca (kallar grass straw) using thermostable cellulases |
Authors: | Latif, Farooq Malik, Dr. Kauser .A. Rajoka, M.Ibrahim |
Keywords: | cellulases Saccharification lignocellulose kallar grass straw thermostable |
Issue Date: | 1994 |
Publisher: | ELSEVIER ( science direct ) |
Citation: | Farooq Latif, M. Ibrahim Rajoka, Kauser A. Malik, Saccharification of Leptochloa fusca (kallar grass straw) using thermostable cellulases, Bioresource Technology, Volume 50, Issue 2, 1994, Pages 107-111, ISSN 0960-8524, https://doi.org/10.1016/0960-8524(94)90061-2. |
Series/Report no.: | Bioresource Technology, Volume 50, Issue 2, 1994, Pages 107-111, ISSN 0960-8524,; |
Abstract: | Saccharifying ability of thermostable cellulases on kallar grass straw at 50 and 60°C was evaluated. Enzyme filtrate from Chaetomium thermophile saccharified 5% kallar grass straw to 69% reducing sugars (quantitatively) at 50°C. Culture filtrates from all the thermophilic fungi showed a considerable saccharification rate up to 40 h. Freeze-dried enzyme of Trichoderma reesei VTT-D-79125 (mesophile), even at higher enzyme concentration resulted in 60% reducing sugars yield (quantitatively). Glucose concentration in the hydrolysates from the fungi was in the order of C. thermophile > T. reesei > Sporotrichum thermophile > Aspergillus fumigatus > Torula thermophila > Humicola grisea > Malbranchea pulchella. At 60°C, thermostable enzymes hydrolysed kallar grass straw at a maximum rate for the initial 20 h, after which the rate essentially stopped increasing. However, at elevated temperature C. thermophile enzymes saccharified kallar grass straw up to 20 h to almost the same yield in half the time as compared to that at 50°C. The overall decline in saccharification yield (11%) of C. thermophile enzymes was significantly lower than T. reesei enzymes (34%) at 60°C. |
Description: | https://www.sciencedirect.com/science/article/abs/pii/0960852494900612 |
URI: | http://localhost:8080/xmlui/handle/123456789/1305 |
Appears in Collections: | School of Life Sciences |
Files in This Item:
File | Description | Size | Format | |
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Saccharification of Leptochloa fusca (kallar grass straw) using thermostable cellulases.pdf | 2.49 MB | Adobe PDF | View/Open |
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