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http://digitalrepository.fccollege.edu.pk/handle/123456789/1316
Title: | Mutagenesis of Cellulomonas biazotea for enhanced production of xylanases |
Authors: | Rajoka, M.Ibrahim Malik, Dr. Kauser .A. Bashir, Dr. Aftab |
Keywords: | cellulases Xylanase β-xylosidase pretreatment carbohydrates protein Cellulomonas production induction lignocellulose kallar grass straw thermostable |
Issue Date: | Dec-1997 |
Publisher: | ELSEVIER |
Citation: | M.Ibrahim Rajoka, A. Bashir, Kauser A. Malik, Mutagenesis of Cellulomonas biazotea for enhanced production of xylanases, Bioresource Technology, Volume 62, Issue 3, 1997, Pages 99-108, ISSN 0960-8524, https://doi.org/10.1016/S0960-8524(97)00116-8. |
Series/Report no.: | Bioresource Technology, Volume 62, Issue 3, 1997, Pages 99-108, ISSN 0960-8524, https://doi.org/1; |
Abstract: | A rifampin-resistant mutant of Cellulomonas biazotea, designated 7 Rf, secreted elevated levels of xylanases in vivo. The xylanase and β-xylosidase production in the mutant was not inhibited in the presence of 5% glucose, cellobiose or glycerol in the solid medium. This mutant showed approximately 2–3-fold enhanced product yield and productivity of cellular β-xylosidase over the wild type parent in shake flask culture studies when grown on either cellobiose, cellulosic or lignocellulosic (LC) substrates. Extracellular production of xylanase was also significantly (P ≤ 0.05) altered in the mutant. During growth of C. biazotea mutant derivative on xylan, maximum volumetric productivities (Qp) for xylanase and β-xylosidase were 493, and 30.7 IU/l/h, which were 1.21- and 2.29-fold improved over their respective values from the parental strain. The above values are statistically (P ≤ 0.05) higher than the values reported in some other xylanolytic mutant organisms. |
Description: | https://www.sciencedirect.com/science/article/abs/pii/S0960852497001168 |
URI: | http://localhost:8080/xmlui/handle/123456789/1316 |
Appears in Collections: | School of Life Sciences |
Files in This Item:
File | Description | Size | Format | |
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Mutagenesis of Cellulomonas biazotea for enhanced production of xylanases.pdf | 4.99 MB | Adobe PDF | View/Open |
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