Cloning of structural genes for ß-glucosidase fromCellulomonas biazotea intoE. coli andSaccharomyces cerevisiae using shuttle vector pBLU-D

Please use this identifier to cite or link to this item: http://digitalrepository.fccollege.edu.pk/handle/123456789/1344

Title:	Cloning of structural genes for ß-glucosidase fromCellulomonas biazotea intoE. coli andSaccharomyces cerevisiae using shuttle vector pBLU-D
Authors:	Rajoka, Muhammad I. Parvez, S. Malik, Dr. Kauser .A.
Keywords:	cloning structural genes biazotea
Issue Date:	Nov-1992
Publisher:	springer link
Citation:	Rajoka, M.I., Parvez, S. & Malik, K.A. Cloning of structural genes for ß-glucosidase fromCellulomonas biazotea intoE. coli andSaccharomyces cerevisiae using shuttle vector pBLU-D. Biotechnol Lett 14, 1001–1006 (1992). https://doi.org/10.1007/BF01021048
Series/Report no.:	Biotechnol Lett 14, 1001–1006 (1992).;
Abstract:	A Smal genomic library fromCellulomonas biazotea NIAB 442 was constructed inEscherichia coli HB101 using shuttle vector pBLU-D. Three clones with ability to hydrolyse esculin were isolated. These clones were comparedin vivo andin vitro tests to select for hyper-secretion of ß-glucosidase. The recombinant plasmids were transformed to competent cells of a Ciro yeast.In vivo studies indicated that the genes were fully expressed in yeast as well.
Description:	https://doi.org/10.1007/BF01021048
URI:	http://localhost:8080/xmlui/handle/123456789/1344
Appears in Collections:	School of Life Sciences

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