Please use this identifier to cite or link to this item:
http://digitalrepository.fccollege.edu.pk/handle/123456789/263
Title: | Stable transformation and expression of GhEXPA8 fiber expansin gene to improve fiber length and micronaire value in cotton |
Authors: | Bajwa, K. S. Shahid, Ahmad A. Rao, Abul Q. Bashir, Dr. Aftab Aftab, Aasia Husnain, Tayyab |
Keywords: | Biological Sciences |
Issue Date: | 2015 |
Publisher: | frontiersin.org |
Citation: | https://www.frontiersin.org/articles/10.3389/fpls.2015.00838/full |
Series/Report no.: | 6(838);1-13 |
Abstract: | Cotton fiber is multigenic trait controlled by number of genes. Previous studies suggest that one of these genes may be responsible for switching cotton fiber growth on and off to influence the fiber quality produced from a cotton seed. In the present study, the Gossypium hirsutum GhEXPA8 fiber expansin gene was introduced into local cotton variety NIAB 846 by using an Agrobacterium-mediated gene transformation. The neomycin phosphotransferase (NPTII) gene was used as a selection marker for screening of putative transgenic cotton plants. Integration and expression of the fiber expansin gene in cotton plants was confirmed with molecular techniques including Southern blot analyses, real-time PCR. Cellulose assay was used for measurement of cellulose contents of transgenic cotton fiber. The data collected from 3 years of field performance of the transgenic cotton plants expressing GhEXPA8 showed that significant improvement has been made in fiber lengths and micronaire values as compared to control G. hirsutum variety NIAB 846 cotton plants. Statistical techniques were also used for analysis of fiber and agronomic characteristics. The results of this study support improvement of cotton fiber through genetic modification. |
URI: | http://localhost:8080/xmlui/handle/123456789/263 |
Appears in Collections: | School of Life Sciences |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Research Document.pdf Restricted Access | 86.41 kB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.